HCM250 MRS Agar
$25.30-39
Product No.Product CategorySpecificationHCM250Dehydrated Culture Medium500g/bottle Intended Use For the enrichment, cultivation and isolation of all species of Lactobacillus from all type……
Introduction
| Product No. | Product Category | Specification |
| HCM250 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material.
Principle and Interpretation
Peptone, beef extract powder, yeast extract powder to provide a nitrogen source, vitamins, growth factors; ammonium citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate to provide the training of various lactic acid bacteria growth factor, agar as medium coagulant.
Formulation
| Ingredients | /liter |
| Enzymatic Digest of Casein | 10.0g |
| Meat Extract | 10.0g |
| Yeast extract | 4.0g |
| Triammonium Citrate | 2.0g |
| Sodium Acetate | 5.0g |
| Magnesium Sulphate Heptahydrate | 0.2g |
| Manganese Sulphate Tetrahydrate | 0.05g |
| Dipotassium Hydrogen Phosphate | 2.0g |
| Glucose | 20.0g |
| Polyoxyethylenesorbitan Monooleate | 1.08g |
| Agar | 15.5g |
| pH5.7±0.2 at 25°C | |
Preparation
Suspend 69.8g in 1 L of distilled or deionized water ,heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 °C for 15min.
Quality Control
Cultural characteristics observed after an incubation at 70-74°C for 46~50h
| Quality control strains | Growth |
| Lactobacillus plantarum ATCC 8014 | grows well,white, circular |
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| Bifidobacterium breve ATCC15700 |
Storage and Shelf Life
2-30°C, keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
