HCM175 Tryptone Glucose Extract Agar
$23-31
Product No.Product CategorySpecificationHCM175Dehydrated Culture Medium500g/bottle Intended Use For bacterial counting and cultivation in water, milk, and other dairy products. Principle a……
Introduction
| Product No. | Product Category | Specification |
| HCM175 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For bacterial counting and cultivation in water, milk, and other dairy products.
Principle and Interpretation
Tryptone and beef extract provide nitrogen sources, vitamins, and growth factors. Glucose providescarbon source. Agar is a coagulant.
Formulation
| Ingredients | /liter |
| Tryptone | 5.0g |
| Beef extract | 3.0g |
| Glucose | 1.0g |
| Agar | 15.0g |
| pH 7.0±0.2 at 25°C | |
Preparation
Suspend 24.0g in 1 L distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes.
Quality Control
Cultural characteristics after 24 hours at 35-37°C.
| Quality control strains | Recovery |
| Escherichia coli ATCC25922 | PR≥0.7 |
| Staphylococcus aureusATCC6538 | |
| Pseudomonas aeruginosa ATCC27853 | |
| Bacillus subtilisATCC6633 |
Storage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
