HCM120 Raka-Ray Agar Base
$47-57
CRM012 金黄色葡萄球菌显色培养基说明书 Product No.Product CategorySpecificationHCM120Dehydrated Culture Medium500g/bottle Intended Use For the detection of lactic acid bacteria in beer and……
Introduction
CRM012 金黄色葡萄球菌显色培养基说明书
| Product No. | Product Category | Specification |
| HCM120 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For the detection of lactic acid bacteria in beer and for monitoring inprocess beer quality.
Principle and Interpretation
Yeast extract , tryptone, and liver concentrate , as well as N-acetyl glucosamine, provide nitrogen sources, vitamins, and growth factors. Maltose, fructose, and dextrose are fermentable sugars. Betaine hydrochloride, diammonium hydrogen citrate, and potassium phosphate act as buffers. Potassium aspartate and potassium glutamate supply aspartic acid and glutamic acid. Magnesium sulfate and manganese sulfate supply cations, which are beneficial to the growth of lactic acid bacteria. Agar is a solidifying agent for the culture medium. Additionally, the supplementary reagent 2-phenylethanol added inhibits Gram-negative bacteria, and cycloheximide inhibits yeasts and molds.
Formulation
| Ingredients | /liter |
| Yeast Extract | 5.0 g |
| Tryptone | 20.0 g |
| Liver Concentrate | 1.0 g |
| Maltose | 10.0 g |
| Fructose | 5.0 g |
| Dextrose | 5.0 g |
| Betaine hydrochloride | 2.0 g |
| Diammonium Hydrogen Citrate | 2.0 g |
| Potassium Aspartate | 2.5 g |
| Potassium Glutamate | 2.5 g |
| Magnesium Sulphate | 0.98 g |
| Manganese Sulphate | 0.42 g |
| Potassium Phosphate | 2.0 g |
| N-Acetyl Glucosamine | 0.5 g |
| Agar | 17.0 g |
| pH5.4±0.2 at 25°C | |
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Preparation
1. Weigh 75.9 g of the dry powder of this product, add 1 L of distilled water or deionized water, stir and heat to boil until it is completely dissolved. Then add 10 ml of Tween-80 and 10 vials of accessory reagent A for SR0510 (each vial needs to be dissolved in 1 ml of deionized water first), and sterilize at 121°C for 15 minutes.
2. When the culture medium cools down to around 50 °C, aseptically add 10 vials of accessory reagent B for SR0510 into the culture medium, and mix well for later use.
3. Transfer 0.1 mL of the beer sample to the plate, and then add 15 – 17 mL of RAKA-RAY AGAR. Wait until the culture medium solidifies.
4. Turn the plate upside down and place it in a constant temperature incubator. Incubate at 36 ± 1 °C for 18 – 24 hours; then observe the results.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 18-24 hours
| Quality control strains | Approx. Inoculum(CFU) | Recovery | Characteristics |
| Lactobacillus brevis ATCC14869 | 10 – 100 | PR≥0.5 | Cream-colored colonies |
| Pediococcus acidilactici CICC20791 | 10 – 100 | PR≥0.5 | Cream-colored colonies |
| Saccharomyces cerevisiae ATCC9763 | > 104 | G≤1 | – |
| Escherichia coli ATCC25922 | > 104 | G≤1 | – |
Storage and Shelf Life
2-30°C, keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
