HCM049 Dehydrated Culture Medium 500g/bottle

Intended Use

For cultivation and isolation of yeasts and molds as well as the absence test for Candida albicans.

Principle and Interpretation

Dextrose as carbon source; Mixture of peptic digest of animal tissue and pancreatic digest of casein provide nitrogen; agar as medium coagulant

Formulation

Preparation

Suspend 65g in 1L of distilled water, stirring heated to boiling to completely dissolve ,autoclave at 121°C for 15 minutes.

Quality Control

Cultural characteristics observed after incubation at 30-35°C for 24-48 hours.

Storage and Shelf Life

2-30°C，Keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.

Ingredients	/liter
Mixture of peptic digest of animal tissue and pancreatic digest of casein	10.0g
Dextrose	40.0g
Agar	15.0g
pH5.6±0.2 at 25°C

Quality control strains	Expected Results	Characteristics
Candida albicans ATCC10231	Good, PR≥0.7	ivory colony
Aspergillus niger ATCC16404		white hyphae, balck spore

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Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

European Pharmacopoeia 8.0 (2014): 2.6.13. Microbiological examination of non-sterile products.Guidance for Industry (2004): Sterile Drug Products Produced by Aseptic Processing -Current Good Manufacturing Practice. ISO 11133:2014: Microbiology of food and animal feed and water – Preparation,production, storage and performance testing of culture media.

United States Pharmacopoeia 38 NF 31 (2015): <61> Microbiological examination of non-sterile products: Microbial Enumeration tests.