CRM020B Multiple Enzyme Substrate Medium
$325-500
Product No.Product CategorySpecificationCRM020BDehydrated Culture Medium500g/bottle Intended Use For the determination of total bacterial count in drinking water and water sources. Princip……
Introduction
| Product No. | Product Category | Specification |
| CRM020B | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For the determination of total bacterial count in drinking water and water sources.
Principle and Interpretation
Glucose, peptone, yeast extract, and anhydrous magnesium sulfate provide carbon/nitrogen sources, vitamins, and growth factors.4-Methylumbelliferyl phosphate (MUP) and 4-Methylumbelliferyl-β-D-glucoside (MUG) act as chromogenic enzyme substrates.
Formulation
| Ingredients | /liter |
| Glucose | 1.25 g |
| Anhydrous magnesium sulfate | 0.2 g |
| Peptone | 5.0 g |
| Yeast extract | 3.5 g |
| 4-Methylumbelliferyl phosphate (MUP) | 0.0375 g |
| 4-Methylumbelliferyl-β-D-glucoside (MUG) | 0.03 g |
| pH 7.0±0.2 at 25°C | |
Preparation
Weigh 1.0 g of the medium and dissolve in 100 mL of purified water.Stir until fully dissolved.Filter-sterilize using a 0.22 μm membrane filter.Store at 2–8°C until use.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
| Quality control strains | Approx. Inoculum(CFU) | Characteristics |
| Escherichia coli ATCC 25922 | 10-100 | Turbid growth; fluorescence under 366 nm UV light |
| Staphylococcus aureus ATCC 6538 |
Storage and Shelf Life
2-8℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
