Microbial Detection in Food Safety: Plate Count Method vs. Flow Cytometry
News 18 8 月, 2025
Food safety relies heavily on microbial detection, which plays a critical role in assessing contamination levels, identifying hazards, and ensuring compliance with testing requirements. Selecting the right detection method depends on the type of microorganism, the food matrix, and the goals of the analysis. Among various approaches—including traditional…
Chromogenic Culture Media: Decoding the “Secret Language” of Microorganisms with Color
News 18 8 月, 2025
Principle of Chromogenic Culture Media Chromogenic culture media enable precise microbial identification through enzyme-substrate reactions. When microorganisms grow on the medium, specific enzymes interact with chromogenic substrates—composed of a chromophore and a metabolizable component. This reaction releases a colored compound, making colonies visi…
Pseudomonas aeruginosa ATCC 27853: A Standard Strain for Microbiology Research and Quality Control
News 18 8 月, 2025
IntroductionPseudomonas aeruginosa ATCC 27853 is a widely used reference strain in microbiology, applied in scientific research, quality control, and education. Due to its well-defined characteristics, it is one of the most important strains for studying bacterial physiology, antibiotic susceptibility, and environmental detection. 1. Basic Informati…
HCM072 Cooked Meat Medium Base
Dehydrated Culture Media, News 4 8 月, 2025
Product No.Product CategorySpecificationHCM072Dehydrated Culture Medium500g/bottle Intended Use For the primary growth and maintenance of aerobic and anaerobic organisms. Principle and Interpretation Proteose peptone provide carbon and nitrogen sources, vitamins, and growth factors; Dextrose is the carbon source which can be used as an energy s…
HCM006 Violet Red Bile Glucose Agar
Dehydrated Culture Media, News, Product 4 8 月, 2025
For detection and enumeration of Enterobacteriaceae. Principle and Interpretation Gelatin peptone and yeast extract provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other nutrients essential for bacterial metabolism. This media is selective due to presence of the inhibitors; bile salts positive organisms especially St…
Preparation of Competent E. coli Cells and Plasmid Transformation
News 16 7 月, 2025
Competent Escherichia coli (E. coli) cells are host cells made receptive to foreign DNA by calcium chloride (CaCl₂) treatment, which temporarily increases cell membrane permeability. This process enables efficient plasmid transformation. 1. What Are Competent Cells? Competence is a physiological state that allows cells to uptake and incorporate …
Internal Quality Control of Aseptic Rooms in Food Microbiology Labs
News 16 7 月, 2025
The aseptic room is the cornerstone for testing bacteria, yeast, mold, and various pathogenic microorganisms in food microbiology. Whether it meets sterile operational standards and has a sound internal quality control system directly impacts the accuracy of microbiological testing results. 1. Layout and Design of the Aseptic Room The walls, floo…
Observation of Yeast Morphology and Identification of Live/Dead Cells
News 16 7 月, 2025
This topic may strike a chord with many. Recently, the internet was abuzz with discussions about “fake yeast”—a term that shocked many and highlighted the unseen risks in microbial products. Some so-called “fake yeast” products were allegedly mixed with unknown “carriers”—substances with little to no effect—resulting in diluted y…
Join HuanKai Biology at Lab Asia 2025!
News 14 7 月, 2025
We are pleased to announce that HuanKai Biology will be exhibiting at Lab Asia 2025, one of the leading international platforms for laboratory professionals, researchers, and innovators across Asia and beyond. About Lab Asia Lab Asia is a premier exhibition dedicated to laboratory science and technology. It serves as a hub for professionals to exp…
Key Control Points for Salmonella Detection in the Laboratory
News 5 6 月, 2025
1. Laboratory Setup Salmonella detection must be conducted in a Biosafety Level 2 (BSL-2) laboratory equipped with a biosafety cabinet, especially during biochemical and serological identification of suspected colonies. Always record biosafety cabinet use to maintain traceability and ensure safety compliance. 2. Media, Reagents, and Serums All cul…
Principle and Influencing Factors of Moist Heat Sterilization
News 8 5 月, 2025
Principle of Moist Heat Sterilization: Moist heat sterilization works by causing denaturation of proteins and nucleic acids in microorganisms, leading to their death. This denaturation begins with the breaking of hydrogen bonds, which disrupts the internal structure of proteins and nucleic acids, rendering them non-functional. Moist heat sterilizatio…
Causes of Cytoplasmic Vacuoles in Cell Culture and Solutions
News 8 5 月, 2025
Many researchers encounter cytoplasmic vacuolization during cell culture. But what causes vacuoles to form in cells, and how can we prevent it? What Are Cytoplasmic Vacuoles? Cytoplasmic vacuoles are bubble-like structures visible under a light microscope, showing optical differences from the cytoplasm. The process of vacuole formation is called …