HCM029 Dehydrated Culture Medium 500g/bottle

Intended Use

For the isolation and enumeration of the vegetative and spore forms of Clostridium perfringens in foodstuffs and other materials.

Principle and Interpretation

Peptone and beef extract powder provide nitrogen, vitamins and growth factors; D- mannitol as fermentable sugars; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant;phenol red as a pH indicator; yolk contains lecithin, lecithin to produce Bacillus cereus enzyme recipitation ring around the colonies; and D- mannitol produce acid fermentation Colonies was yellow; polymyxin B inhibit the growth of bacteria.

Formulation

Ingredients	/liter
Beef extract	1.0g
Peptone	10.0g
D- mannitol	10.0g
Sodium chloride	10.0g
Phenol red	0.025 g
Agar	15.0g
pH7.2±0.2 at 25°C

Preparation

Suspend 46g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°Cfor 15minutes and then cool to 50°C. Add 5mL of 50% sterile egg- yolk emulsion(029250) and 10000IU of polymyxin B(SR0170) to 100mL of the medium. Mix thoroughly and pour into sterile Petri dishes.

Quality Control

Cultural characteristics observed after an incubation at 35-37°C for 24 hours

Quality control strains	Growth	Characteristics
Bacillus cereus CMCC(B)63303	Good， PR≥0.7	Pink colonies with precipitation halo
Escherichia coli ATCC25922	none	/

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Storage and Shelf Life

2-30°C，Keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

BAM Media M95 ISO 7932
ISO 21871