Product No.	Product Category	Specification
HCM194	Dehydrated Culture Medium	500g/bottle

Intended Use

For the detection and counting of coliform bacteria.

Principle and Interpretation

Peptone provides nitrogen sources, vitamins and growth factors. Bile salts inhibit the growth of Gram-positive bacteria. Lactose provides carbon source. Neutral red was used to detect lactose-positive colonies, and the lactose-positive colonies were red. Agar is a coagulant.

Formulation

Ingredients	/liter
Peptone	20.0g
Lactose	10.0g
Bile salts	5.0g
Neutral red	0.075g
Agar	12.0g
pH 7.4±0.2 at 25°C

Preparation

Suspend 47.0g in 1 L distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes.

Quality Control

Cultural characteristics after 24 hours at 35-37°C.

Quality control strains	Standard value	Characteristic reaction
Escherichia coli ATCC25922	PR≥0.7	GrowGood, red colonies
Salmonella typhimurium ATCC14028		GrowGood, colorless colonies
Proteus mirabilis ATCC12453	–	GrowGood, colorless colonies,no swarming
Enterococcus faecalisATCC29212	The first area has a small amount of growth or no growth	–

Storage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.