HCM185 Modified Giolitti-Cantoni Broth Base
$39-60
Product No.Product CategorySpecificationHCM185Dehydrated Culture Medium500g/bottle Intended Use Giolitti-Cantoni Broth Base is used detection of coagulase-positive staphylococci in food a……
Introduction
| Product No. | Product Category | Specification |
| HCM185 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
Giolitti-Cantoni Broth Base is used detection of coagulase-positive staphylococci in food and animal feeding stuffs by MPN technique. (ISO)
Principle and Interpretation
Enzymatic digest of casein, meat extract and yeast extract provide nitrogen source, a carbon source and growth factors . Mannitol serves as a fermentable carbon source. Sodium chloride maintains the osmotic pressure. Lithium chloride and glycine inhibit Gram-positive bacteria. Tellurite inhibits Gram-negative bacilli. Sodium pyruvate promotes the growth of staphylococcus. Tween 80 iss used to promote cellular recovery of damaged Staphylococcus aureus.
Formula
| Ingredients | /liter |
| Enzymatic digest of casein | 10.0g |
| Meat extract | 5.0g |
| Yeast extract | 5.0g 5.0g |
| Lithium chloride | 5.0g |
| Mannitol | 20.0g |
| Sodium chloride | 5.0g |
| Glycine | 1.2g |
| Sodium pyruvate | 3.0g |
| Tween 80 | 1.0g |
| pH 6.9±0.2 at 25°C | |
Preparation
Single Strength: Suspend 55.2g in 1 L purified water. Heat with frequent agitation and boil to completely dissolve the powder. Dispense 9 mL into each tube. Autoclave at 121°C for 15 minutes. Then cool to room temperature. Add 0.1mL of 1% Potassium Tellurite (029380) per tube.
Double Strength: Suspend 110.4g in 1 L purified water. Heat with frequent agitation and boil to completely dissolve the powder. Dispense 10 mL into each tube. Autoclave at 121°C for 15 minutes. Then cool to room temperature. Add 0.2mL of 1% Potassium Tellurite (029380) per tube.
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Quality Control
Cultural characteristics after 24-48h hours at 35±2°C.
Storage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
Mar. 1, 2025
References
ISO 6883-3-2003 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci ( Staphylococcus aureus and other species )—Part 3: Detection and MPN technique for low number
