Product No.	Product Category	Specification
HCM092	Dehydrated Culture Medium	500g/bottle
023110	Dehydrated Culture Medium	250g/bottle

Intended Use

For isolating Salmonella other than S. typhi.

Principle and Interpretation

Brilliant green inhibits gram-positive bacteria and a majority of gram-negative bacilli. Phenol red serves as a pH indicator and yields a yellow color as a result of acid production in the fermentation of the lactose and/or sucrose in the medium.

Formulation

Ingredients	/liter
Meat extract	5.0 g
Peptone	10.0 g
Yeast extract	3.0 g
Disodium hydrogen phosphate	1.0 g
Sodium dihydrogen phosphate	0.6 g
Lactose	10.0 g
Sucrose	10.0g
Phenol red	0.09 g
Brilliant green	0.005 g
Agar	15.0 g
PH 7.0±0.2 at 25°C

Preparation

Weigh54.7g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until

completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and

set aside.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Standard value	Characteristic reaction
Salmonella typhimurium ATCC14028	PR≥0.5	Colorless colonies
Escherichia coli ATCC25922	G≤3	_
Enterococcus faecalis ATCC29212		–

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO6785：2001 Milk and milk products—Detection of Salmonella spp.

ISO 3565：1975

ISO 6579-1981