HCM059 Bismuth Sulfite Agar
$199
Product No.Product CategorySpecificationHCM059Dehydrated Culture Medium500g/bottle Intended Use For selective isolation of Salmonella spp, especially for Salmonella typhi. Principle and In……
Introduction
| Product No. | Product Category | Specification |
| HCM059 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For selective isolation of Salmonella spp, especially for Salmonella typhi.
Principle and Interpretation
Peptone, beef extract powder provides carbon, nitrogen, vitamins and minerals; glucose to provide energy; bismuth sulfite indicator suppresses gram-positive bacteria and coliform bacteria, but does not affect the growth of salmonella; agar as culture medium coagulant.
Formulation
| Ingredients | /liter |
| Peptone | 10.0g |
| Beef extract | 5.0g |
| Dextrose | 5.0g |
| FeSO4 (anhydrous) | 0.3g |
| Na2HPO4 (anhydrous) | 4.0 g |
| Bismuth sulfite (indicator) | 8.0 g |
| Brilliant green | 0.025 g |
| Agar | 15.0 g |
| pH7.7±0.2 at 25°C | |
Preparation
Suspend 47.3g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Pour into sterile Petri dishes.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 40~48 hours
| Quality control strains | Growth | Typical reaction |
| Salmonella enterica subsp. Enterica ATCC14028 | Good | Black or gray green colonies, may have a metallic luster |
| Salmonella enterica subsp. enterica serovar Typhi CMCC(B)50071 | Good | Black colonies with metallic luster |
| Escherichia coli ATCC25922 | Marked Inhibition | – |
| Enterococcus faecalis ATCC29212 | Marked Inhibition | – |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
AOAC (2005): Official Method 467.25 Salmonella in Foods. Preparation of Culture Media and Reagents.
APHA (2018): Part 9260: Detection of pathogenic bacteria. Standard Methods for the Examination of Water. 23rd ed.
ISO International Standardization Organization. EN ISO 6579-1:2027/ Amd1:2020.
FDA-BAM (2018): Media Index for BAM – BAM Media M19: Bismuth Sulfite Agar (Wilson and Blair). Food and Drug Administration – Bacteriological Analytical Manual.
United States Pharmacopeial Convention. (2008): The United States Pharmacopeia 31/National Formulation 26. Chapter <61> Microbial limit test.
