HCM020 Thiosulfate Citrate Bile and Sucrose Agar (TCBS) Agar
$23-34
Intended Use For the cultivation of various fastidious, pathogenic microorganisms including yeasts and molds. Principle and Interpretation This culture medium contains the selective compon……
Introduction
Intended Use
For the cultivation of various fastidious, pathogenic microorganisms including yeasts and molds.
Principle and Interpretation
This culture medium contains the selective components bile salts, thiosulfate, citrate and are latively high pH of 8.6. The high concentrations of thiosulfate and citrate and the strong alkalinity of this medium largely inhibit the growth of Enterobacteriaceae. Ox bile and cholate suppress primarily enterococci. Differentiation is based on sucrose fermentation, detected by the pH indicators, and H2S production from thiosulfate. The combination of thiosulfate as a reactive compound with an iron salt as an indicator causes H2S-positive colonies to become black in color. Vibrio species do not produce H2S but, depending on the species, may ferment sucrose .Any coliform bacteria, which may grow, cannot metabolize sucrose. Only a few sucrose-positive Proteus strains can grow to form yellow, vibrioid-like colonies. The mixed indicator thymol blue bromothymol blue changes its color to yellow, when acid is formed, even in this strongly alkaline medium. Peptone and yeast extract allow good growth and agar is the solidifying agent.
Formulation
| Product No. | Product Category | Specification |
| HCM020 | Dehydrated Culture Medium | 500g/bottle |
| Ingredients | /liter |
| Peptone | 10.0g |
| Yeast extract | 5.0g |
| Sodium citrate | 10.0g |
| Sodium thiosulfate | 10.0g |
| Iron (III) citrate | 1.0g |
| Sodium cholate | 10.0g |
| Dried bovine bile | 8.0g |
| Sucrose | 20.0g |
| Bromothymol blue | 0.04g |
| Thymol blue | 0.04g |
| Agar | 15.0g |
| pH8.6±0.2 at 25°C | |
Preparation
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Dissolve 89.0 g in 1 liter of purified water. Heat in boiling water and agitate frequently until completely dissolved. Do not autoclave!.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24hours
Storage and Shelf Life
2-30°C, keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
ISO 21872
BAM Media M147
