Intended Use

For the cultivation of various fastidious, pathogenic microorganisms including yeasts and molds.

Principle and Interpretation

Peptone and Dehydrated calf brain infusion and Dehydrated beef heart infusion provide nitrogen sources, vitamins, and growth factors; Glucose can provide energy for various bacteria; Sodium chloride maintains a balanced osmotic pressure; Sodium dihydrogen phosphate as a buffering agent.

Formulation

Ingredients	/liter
Dehydrated calf brain infusion	12.5g*
Dehydrated beef heart infusion	5.0g**
Enzymatic digest of animal tissues (Peptone)	10.0g
Sodium chloride	5.0g
Glucose	2.0g
Disodium hydrogen phosphate	2.5g
pH7.4±0.2 at 25°C

*Equivalent to calf brain, infusion from 200g, ** Equivalent to beef heart, Infusion from 250g.

Preparation

Dissolve 37 g in 1 liter distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 24hours

Quality control strains	Growth
Staphylococcus aureus  ATCC6538	good

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 6888-1 2020

BAM Media M24