Introduction
Usages:
For isolation and enumeration of Pseudomonas aeruginosa in water samples.
Principle:
Peptone provide nitrogen; glycerol carbon source; potassium and magnesium can promote green pus pigment production; hexadecyl trimethyl ammonium bromide is a selective antibacterial agent, as a quaternary ammonium salt cationic detergent may bacterial cells release nitrogen and phosphorus and suppress non-Pseudomonas aeruginosa bacteria; agar is medium coagulant.
Formulation（per liter）:
Pancreatic Digest of Peptone 20.0g
Magnesium Chloride 1.4g
Dipotassium Sulphate 10.0g
Cetrimide 0.3g
Agar 13.6g
Final pH 7.2 ± 0.2
How to use：
1.Suspend 45.3g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control：
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Pseudomonas aeruginosa ATCC27853 | Good | Yellow -green |
| 2 | Escherichia coli ATCC25922 | Inhibition | — |
| 3 | Staphylococcus aureus ATCC25923 | Inhibition | — |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
*Supplement:029080 Glycerol
