027043P1 Cetrimide Agar Base




For isolation and enumeration of Pseudomonas aeruginosa in water samples.

Peptone provide nitrogen; glycerol carbon source; potassium and magnesium can promote green pus pigment production; hexadecyl trimethyl ammonium bromide is a selective antibacterial agent, as a quaternary ammonium salt cationic detergent may bacterial cells release nitrogen and phosphorus and suppress non-Pseudomonas aeruginosa bacteria; agar is medium coagulant.

Formulation(per liter):
Pancreatic Digest of Peptone 20.0g
Magnesium Chloride     1.4g
Dipotassium Sulphate   10.0g
Cetrimide   0.3g
Agar 13.6g
Final pH 7.2 ± 0.2

How to use:
1.Suspend 45.3g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Quality control:

ItemThe name and number of strainGrowthColony Color
1Pseudomonas aeruginosa ATCC27853GoodYellow -green
2Escherichia coli ATCC25922Inhibition
3Staphylococcus aureus ATCC25923Inhibition

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle
*Supplement:029080 Glycerol

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