Pseudomonas aeruginosa ATCC 27853: A Standard Strain for Microbiology Research and Quality Control
News 18 8 月, 2025
Introduction
Pseudomonas aeruginosa ATCC 27853 is a widely used reference strain in microbiology, applied in scientific research, quality control, and education. Due to its well-defined characteristics, it is one of the most important strains for studying bacterial physiology, antibiotic susceptibility, and environmental detection.
1. Basic Information
- Scientific Name: Pseudomonas aeruginosa
- Strain Number: ATCC 27853
- Origin: American Type Culture Collection (ATCC)
- Characteristics:
- Gram-negative, rod-shaped bacterium
- Motile with a single polar flagellum
- Produces pyocyanin and fluorescein, giving colonies a greenish appearance
- Strictly aerobic, with a wide growth temperature range (4°C–42°C), optimal at 37°C
2. Applications
- Microbiology Research: A model strain for studying metabolism, physiology, and pathogenic mechanisms.
- Quality Control: Commonly used in pharmaceutical, food, and cosmetic industries for testing media quality and antibiotic susceptibility.
- Environmental Detection: Useful in water testing, environmental monitoring, and sterilization efficiency assessment of medical devices.
- Teaching: Widely applied in laboratory courses for bacterial culture and identification experiments.
3. Culture Conditions
- Media: Nutrient agar (NA), tryptic soy agar (TSA), or LB agar
- Incubation Temperature: 37°C
- Incubation Time: 24–48 hours
- Atmosphere: Aerobic
4. Preservation Methods
- Short-Term: Store on agar slants at 4°C (weeks to months).
- Long-Term: Glycerol stock (50%) at −80°C (years).
- Freeze-Dried (Lyophilized): Provided by ATCC, stable at 4°C and revived per supplier instructions.
5. Safety Precautions
- P. aeruginosa is an opportunistic pathogen.
- Work should be carried out in a biosafety cabinet with appropriate PPE (gloves, mask).
- Avoid excessive subculturing; revive from lyophilized stock every 3–6 months to maintain strain stability.
6. Research Significance
- Pathogenicity: A major cause of hospital-acquired infections, particularly dangerous to immunocompromised patients.
- Antibiotic Resistance: Known for multidrug resistance, making it an important model in antimicrobial research.
- Biofilm Formation: Capable of forming biofilms, which complicates treatment and promotes chronic infections. Studying this process helps in developing new antibacterial strategies.
Conclusion
Pseudomonas aeruginosa ATCC 27853 plays a critical role in microbiological research and industry quality control. Its well-characterized features make it a reliable reference strain for studying bacterial physiology, resistance, and biofilm mechanisms.