HCM072 Cooked Meat Medium Base
Dehydrated Culture Media, News 4 8 月, 2025
| Product No. | Product Category | Specification |
| HCM072 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For the primary growth and maintenance of aerobic and anaerobic organisms.
Principle and Interpretation
Proteose peptone provide carbon and nitrogen sources, vitamins, and growth factors; Dextrose is the carbon source which can be used as an energy source. Sodium chloride provides the osmotic equilibrium for the bacteria cells.Cooked Meat Particles as denatured protein, it contains more sulfur-hydrogen groups, and provide a good environment for the growth of anaerobic bacteria.
Formulation
| Ingredients | /liter |
| Proteose peptone | 20 g |
| Dextrose | 2 g |
| NaCl | 5 g |
| pH7.2±0.2 at 25°C | |
Preparation
Suspend 27g of the powder in1L of purified water, heat with frequent agitation and boil to completely dissolved the powder. Distribute 10ml into tubes ,then add Cooked Meat Particles (027150) 0.98g,let stand until all particles are thoroughly wetted. Autoclave at 121℃ for 15 minutes.
Quality Control
The following quality control strains were inoculated and cultured under anaerobic conditions at 35-37℃ for 24h. The results are as follows:
| Quality control strains | Property | Growth |
| Clostridium perfringens ATCC13124 | Turbidity 2 | turbid |
| Clostridium sporogenes ATCC19404 |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024