Product No.	Product Category	Specification
CRM022B	Dehydrated Culture Medium	500g/bottle

Intended Use

For isolation and identification of urinary tract pathogens.

Principle and Interpretation

Peptoneand yeast extract supply essential nutrients and vitamins in this medium. Chromogenic mixdetermines the color of different pathogens in genus level. Agar is coagulant.

Formulation

Ingredients	/liter
Peptone	12.0 g
Yeast extract	5.0 g
Chromogenic mix	1.0 g
Agar	15.0 g
pH 7.0±0.2 at 25°C

Preparation

Suspend 33.0g in 1 L distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 18-24 hours

Quality control strains	Approx. Inoculum(CFU)	Recovery	Growth
Escherichia coli ATCC25922	10-100	PR≥0.5	Purple-red colonies
Enterococcus faecalis ATCC29212			Turquoise colonies
Citrobacter fischeri ATCC43864			Metallic blue with red halo colonies
Staphylococcus saprophyticus FSCC(I)223042			Light pink colonies
Staphylococcus aureus ATCC 6538			White to gold colonies
Streptococcus agalactiae ATCC12386			Sapphirine colonies
Pseudomonas aeruginosa ATCC27853			Translucent colonies,may be a faint green pigment
Proteus mirabilis CMCC(B)49005			Brown halo colonies

Storage and Shelf Life

Dry powder was stored in a sealed container at 2-30℃ for two years in a dry environment. The prepared plates were stored at 2-8℃ for one week ,Use before expiry date on the label.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.