Specification

500g/bottle 250g/bottle

Intended Use

For the cultivation of E. coli in fermentation and molecular genetic studies. Principle and Interpretation

Casein peptone and yeast extract supply essential growth factors,such as nitrogen, carbon, sulfur, minerals and vitamins.

Formulation

Preparation

Suspend 20 g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 24 hours

Storage and Shelf Life

2-30°C，Keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Ingredients	/liter
Tryptone	10.0g
Yeast extract	5.0g
Sodium chloride	5.0g
pH7.0±0.2 at 25°C

Quality control strains	Growth
Escherichia coli ATCC25922	good

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Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

Lennox

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort 2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.