Product No.	Product Category	Specification
HCM203	Dehydrated Culture Medium	500g/bottle

Intended Use

For enumeration of Pseudomonas spp of meat and meat products.

Principle and Interpretation

Gelatin peptone and casein hydrolysate provide nitrogen source, vitamins and growth factors. Glycerol provides carbon source. Potassium sulfate and magnesium chloride promote pigment production of Pseudomonas aeruginosa. Agar serves as the solidifying agent.

Formula

Ingredients	/liter
Gelatin peptone	16.0g
Casein hydrolysate	10.0g
Potassium sulfate	10.0g
Magnesium chloride	1.4g
Agar	15.0g
pH 7.1±0.2 at 25°C

Preparation

Suspend 52.4g in 1 L distilled or deionized water. Add 10mL glycerol. Heat with frequent agitation and boil to completely dissolve the powder. Autoclave at 121℃ for 15 minutes. Then cool to 50℃. Add 1 vail of CFC Supplement (SR1120) to 500mL medium base to prepare CFC Agar. Add 1 vail of CN Supplement (SR1130) to 500mL medium base to prepare CN Agar.

Quality Control

CFC Agar: Cultural characteristics after 24-48h hours at 25±1℃.

Quality control strains	Growth
Pseudomonas aeruginosa ATCC27853	Good
Pseudomonas fluorescens ATCC13525
Escherichia coli ATCC25922	Inhibited
Enterococcus faecalis ATCC29212

CN Agar: Cultural characteristics after 40-48h hours at 36±1℃.

Quality control strains	Growth
Pseudomonas aeruginosa ATCC27853	Good
Pseudomonas aeruginosa ATCC9027
Escherichia coli ATCC25922	Inhibited
Enterococcus faecalis  ATCC29212

Storage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.