Product No.	Product Category	Specification
HCM153	Dehydrated Culture Medium	500g/bottle

Intended Use

For the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria.

Principle and Interpretation

Yeast extract and casein enzymic hydrolysate provide nitrogen source, vitamins, and growth factors; glucose is fermentable sugar; potassium dihydrogen phosphate is an acid-base buffer; potassium chloride, calcium chloride, and ferric chloride maintain the osmotic pressure of the culture medium; magnesium sulfate and manganese sulfate provide divalent cations; various positive ions are provided; bromocresol green is an acid-base indicator; agar is a coagulant.

Formulation

Ingredients	/liter
Yeast extract	4.0g
Casein enzymic hydrolysate(Tryptone)	5.0g
Glucose	50.0g
Potassium dihydrogen phosphate	0.55g
Potassium chloride	0.425g
Calcium chloride	0.125 g
Magnesium sulfate	0.125 g
Ferric chloride	0.0025 g
Manganese sulfate	0.0025 g
Bromocresol green	0.022 g
Agar	17.0g
pH5.5±0.2 at 25°C

Preparation

Suspend 77.3g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes..

Quality Control

Cultural characteristics observed after incubation at 30+/-2°C for 48-72hhours

Quality control strains	Inoculum CFU	Expected Result
Saccharomyces cerevisiae ATCC9763	10-100	>50%,green colonies with dark green centres

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

MacFaddin, 1985, Media for isolation-cultivation-identification-maintenance of medical bacteria, Vol. 1,Williams & Wilkins, Baltimore, Md.