HCM144 Modified Phosphate Buffer
$28-45
Peptone Sorbitol Bile Broth Product No.Product CategorySpecificationHCM144Dehydrated Culture Medium500g/bottle Intended Use For the selective enrichment of Yersinia enterocolitica. Prin……
Introduction
Peptone Sorbitol Bile Broth
| Product No. | Product Category | Specification |
| HCM144 | Dehydrated Culture Medium | 500g/bottle |
Intended Use
For the selective enrichment of Yersinia enterocolitica.
Principle and Interpretation
Peptone (enzymatic of digest of casein) and sorbitol provides carbon and nitrogen sources, disodium hydrogen phosphate and sodium dihydrogen phosphate are buffers, bile salts inhibit Gram-positive bacteria, and sodium chloride provides the osmotic pressure required by cells.
Formulation
| Ingredients | /liter |
| Peptone (enzymatic of digest of casein) | 5.0g |
| Sorbitol | 10.0g |
| NaCl | 5.0g |
| Na2HPO4 | 8.23 g |
| NaH2PO4 · H2O | 1.2 g |
| Bile salts No. 3 | 1.5 g |
| pH7.6±0.2 at 25°C | |
Preparation
Suspend 30.93 g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes..
Quality Control
Cultural characteristics observed after incubation at 26±1℃ for 48hhours
| Quality control strains | Inoculum CFU | Expected Result |
| Yersinia enterocolitica CMCC(B)52204 | 10-100 | good growth |
| Enterococcus faecalis ATCC29212 | ≥ 100 | no growth |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
FDA BAM Media M120
ISO 10273：2017
