Product No.	Product Category	Specification
HCM105	Dehydrated Culture Medium	500g/bottle
028361	Dehydrated Culture Medium	250g/bottle

Intended Use

For the cultivation of a wide variety of fastidious organisms.

Principle and Interpretation

The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Glucose is a carbohydrate source that microorganisms utilize by fermentative action. The medium is buffered through the use of disodium phosphate.

Formulation

Ingredients	/liter
Dehydrated calf brain infusion	12.5g*
Dehydrated beef heart infusion	5.0g**
Enzymatic digest of animal tissues (Peptone)	10.0g
Sodium chloride	5.0g
Glucose	2.0g
Disodium hydrogen phosphate	2.5g
Agar	15.0g
pH7.4±0.2 at 25°C

*Equivalent to calf brain, infusion from 200g, ** Equivalent to beef heart, Infusion from 250g.

Preparation

Weigh 52g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, sterilize at 121℃ for 15min, cool to room temperature and set aside.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Standard value	Characteristic reaction
Streptococcus pyogenes ATCC21059	PR≥0.7	Grayish white colonies
Enterococcus faecalis ATCC29212		Grayish white colonies
Escherichia coli ATCC25922		Grayish white colonies

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 6888-1 2020

BAM Media M24