Product No.	Product Category	Specification
HCM091	Dehydrated Culture Medium	500g/bottle
022116	Dehydrated Culture Medium	250g/bottle

Intended Use

For the dissolving, suspending and diluting of test samples from pharmaceutical and other material and of test strains.

Principle and Interpretation

The low peptone provide the nitrogen source, vitamins, amino acids, and carbon to maintain organisms viability, but does not allow significant growth of more fastidious microorganisms; sodium chloride to maintain osmotic balance; potassium dihydrogen phosphate and disodium hydrogen phosphate as a buffer.

Formulation

Ingredients	/liter
Peptone (meat or casein)	1.0 g
Potassium dihydrogen phosphate	3.6 g
Disodium hydrogen phosphate dihydrate	7.2 g
Sodium chloride	4.3 g
pH 7.0±0.2  at 25°C

Preparation

Suspend 16.1 g in 1 litre of purified water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize by autoclaving at 121°C for 15 minutes.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Incubation	Reference medium	Method of control	Expected results
Escherichia coli ATCC8739	45 min at 20-25℃ (room temp.)	Tryptic Soy Agar (TSA)	Quantitative	± 50 % colonies
Staphylococcus aureus ATCC6538

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

USP /EP  Microbiological examination of nonsterile pruducts