Product No.	Product Category	Specification
HCM089	Dehydrated Culture Medium	500g/bottle
022029	Dehydrated Culture Medium	250g/bottle

Intended Use

For the enumeration and cultivation of bacteria from potable water.   

Principle and Interpretation

Casein hydrolysate and peptone provide nitrogen, vitamins, amino acids, carbon and minerals. Yeast extract is a source of vitamins and essential trace elements. Soluble starch has a positive impact on the recovery of injured organisms due to its ability to absorb toxic metabolic by-products. Sodium pyruvate also aids with the recovery of stressed cells. Magnesium sulphate provides divalent cations and sulphate. Potassium phosphate is added to stabilize the pH and as a phosphate source. Agar acts as solidifying agent.

Formulation

Ingredients	/liter
Yeast extract powder	0.5g
Peptone	0.5g
Casein hydrolyzate	0.5g
Glucose	0.5g
Soluble starch	0.5g
Dipotassium hydrogen phosphate	0.3g
Magnesium sulfate, anhydrous	0.024g
Sodium pyruvate	0.3g
Agar	15.0g
pH7.2±0.2 at 25°C

Preparation

Suspend 18.1g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes..

Quality Control

The following quality control strains were inoculated and cultured at 30-35℃ for 24h. The results are as follows:

Quality control strains	Inoculum (CFU)	Expected results
Pseudomonas aeruginosa ATCC9027	10-100	50-200% recovery
Bacillus subtilis ATCC6633

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.