Product No.	Product Category	Specification
HCM028	Dehydrated Culture Medium	500g/bottle

Intended Use

For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material.

Principle and Interpretation

Peptone, beef extract powder, yeast extract powder to provide a nitrogen source, vitamins, growth factors; ammonium citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate to provide the training of various lactic acid bacteria growth factor, agar as medium coagulant.

Formulation

Ingredients	/liter
Peptone	10.0g
Beef extract	5.0g
Yeast extract	4.0g
Glucose	20.0g
Tween -80	1.0mL
Dipotassium hydrogen phosphate	2.0g
Sodium acetate	5.0g
Ammonium citrate	2.0g
Magnesium sulfate	0.2g
Manganese sulfate	0.05g
Agar	15.0g
pH6.2±0.2 at 25°C

Preparation

Suspend 64.3g in 1 L of distilled or deionized water ,heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min.

Quality Control

Cultural characteristics observed after an incubation at 35-37°C for 46~50h

Quality control strains	Growth
Lactobacillus delbrueckii subsp. Bulgaricus CICC6032	PR≥0.7， cream colonies
Streptococcus thermophilus  IFFI6038
Bifidobacterium infantis CICC6069（ Anaerobic culture）

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024