HCM007 Nutrient Agar
$29-48
Intended Use For the cultivation of non-fastidious bacteria. Principle and Interpretation Specification 500g/bottle Peptone and beef extract provide the necessary nitrogen compounds,……
Introduction
Intended Use
For the cultivation of non-fastidious bacteria.
Principle and Interpretation
Specification
500g/bottle
Peptone and beef extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria.
Formulation
Preparation
Suspend 23.0 g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into tubes or flasks. Autoclave at 121°C for 15 minutes.
Quality Control
Cultural characteristics observed after an incubation at 35-37°C for 24 hours
Storage and Shelf Life
2-30°C，Keep container tightly closed, avoid direct sunlight. Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
| Ingredients | /liter |
| Beef extract | 3.0g |
| Peptone | 5.0g |
| Agar | 15.0g |
| pH7.0±0.2 at 25°C | |
| Quality control strains | Growth | Characteristics |
| Escherichia coli ATCC25922 | Good, PR≥0.7 | Off white colony |
| Pseudomonas aeruginosa ATCC27853 | Produce green pigment | |
| Streptococcus faecalis ATCC29212 | Off white little colony |
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2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
FDA BAM.
APHA (2015) Compendium of Methods for the Microbiological Examination of Foods. 5th ed. American Public Health Association, Washington, D.C.
EN.ISO6579:2002. Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Salmonella spp.
EN ISO/FDIS 6579-1:2015. Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Horizontal method for the detection of Salmonella spp.
EN ISO 6785 I IDF 93:2001. Milk and milk products – Detection of Salmonella spp.
EN ISO 19250:2010. Water quality – Detection of Salmonella spp.
EN ISO 10272:2003. Microbiology of food and animal feeding stuffs – Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica.
EN ISO 21528-2004. Microbiology of food and animal feeding stuffs – Horizontal methods for the detection and enumeration of Enterobacteriaceae – Part 2: Colony-count method.
