HCM087 Reinforced Medium for Clostridia
$27-$39
Reinforced Medium for Clostridia– Avaliable in 500g and 250g. Intended Use For the enumeration and cultivation of clostridia and other anaerobes occurring in food and pathological speci……
Introduction
Reinforced Medium for Clostridia– Avaliable in 500g and 250g.
Intended Use
For the enumeration and cultivation of clostridia and other anaerobes occurring in food and pathological specimens.
Principle and Interpretation
Peptone, yeast extract, beef extract , starch, Cysteine hydrochloride and sodium acetate provide all the necessary nutrients for the growth of Clostridia. Glucose is the carbohydrate source. Sodium chloride maintains the osmotic balance. In low concentrations, soluble starch detoxifies metabolic by-products. Cysteine hydrochloride is the reducing agent. Sodium acetate acts as a buffer. The small amount of agar makes the medium semisolid.
Formulation
| Ingredients | /liter |
| Beef extract | 10 g |
| Peptone | 10g |
| Yeast extract | 3.0 g |
| Glucose Monohydrate | 5.0g |
| Sodium chloride | 5.0 g |
| Soluble starch | 1.0 g |
| Cysteine Hydrochloride | 0.5 g |
| Sodium Acetate | 3.0 g |
| Agar | 0.5 g |
| pH 6.8±0.2 at 25°C | |
Preparation
Weigh 38g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside.
Quality Control
The following quality control strains were inoculated and cultured under anaerobic conditions at 30-35℃ for 24-48h. The results are as follows:
| Quality control strains | Inoculum (CFU) | Expected results |
| Clostridium sporogenes ATCC19404 | ≤ 100 | turbid |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
USP Volume <62>Microbiological examination of nonsterile products：test for special microorganisms
The European Pharmacopoeia. 8th Ed. Chapter 2.6.13 Microbiological examination of non-sterile products: Test for specified products.
