Kligler Iron Agar





For differentiation of enterobacteriaceae.


Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.

Formulation(per liter):

Peptone 20g

Beef extract powder 3g

Yeast extract powder 3g

Lactose 10g

Dextrose 1g

Sodium chloride 5g

Ferric ammonium citrate 0.5g

Sodium thiosulfate 0.5g

Agar 12g

Phenol red 0.05g

Final pH 7.4 ± 0.2

How to use:

1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.

2.Diluted and treated samples.

Quality control:

ItemThe name and number of strainGrowthColony Color
1Escherichia coli ATCC25922GoodA/A
2Proteus CMCC (B) 49027GoodK/A 
3Salmonella typhimurium CMCC (B) 50115GoodK/A

“A”Acid , “K” Alkality

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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